The forthcoming soundtrack also features DJ Shadow and De La Soul.
MC Hammer leads a summer US trek with assistance from Sir Mix-a-Lot, Sisqo, En Vogue, Coolio, and more.
A Target commercial featuring the Mike WiLL Made-It-produced rendition will air during the Grammys on Sunday.
Fallon and the Fresh Prince beatbox AND rap the hip-hop classic.
Harlem native was a frequent collaborator of Rob Base.